Published June 2018
| public
Journal Article
Microfluidics and single-cell microscopy to study stochastic processes in bacteria
Abstract
Bacteria have molecules present in low and fluctuating numbers that randomize cell behaviors. Understanding these stochastic processes and their impact on cells has, until recently, been limited by the lack of single-cell measurement methods. Here, we review recent developments in microfluidics that enable following individual cells over long periods of time under precisely controlled conditions, and counting individual fluorescent molecules in many cells. We showcase discoveries that were made possible using these devices in various aspects of microbiology, such as antibiotic tolerance/persistence, cell-size control, cell-fate determination, DNA damage response, and synthetic biology.
Additional Information
© 2017 Published by Elsevier. Available online 27 February 2018. This review comes from a themed issue on The new microscopy. Edited by Ariane Briegel and Stephan Uphoff. This work was supported by DARPA grant HR0011-16-2-0049, NIH grants (GM081563 and GM095784), and NSF award 1517372.Additional details
- PMCID
- PMC6044433
- Eprint ID
- 88307
- Resolver ID
- CaltechAUTHORS:20180726-141914171
- HR0011-16-2-0049
- Defense Advanced Research Projects Agency (DARPA)
- GM081563
- NIH
- GM095784
- NIH
- DMS-1517372
- NSF
- Created
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2018-07-26Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field