Orienting DNA Helices on Gold Using Applied Electric Fields
Abstract
Gold surfaces modified with thiol-derivatized DNA duplexes have been investigated as a function of applied electrochemical potential via atomic force microscopy (EC-AFM). At open circuit, monolayers of well-packed DNA helices form with a film depth of 45(3) Å. On the basis of the anisotropic dimensions of these 15 base pair duplexes (20 Å in diameter versus 50 Å in length), this corresponds to an average ∼45° orientation of the helical axis with respect to the gold surface. Under potential control, the monolayer thickness (and therefore the orientation of the helices) changes dramatically with applied potential. At potentials negative of ∼0.45 V (versus a Ag wire quasi-reference electrode) film thicknesses of ∼55 Å are observed, whereas at more positive potentials the monolayer thickness drops to a limiting value of ∼20 Å. These results are consistent with a morphology change in which the helices either stand straight up or lie flat down on the metal surface, depending on the electrode potential relative to the potential of zero charge (pzc). This voltage-induced morphology change is reversible and effectively constitutes a nanoscale mechanical "switch".
Additional Information
© 1998 American Chemical Society. Received July 14, 1998. In Final Form: September 24, 1998. This work was supported by the NSF (M.G.H. and E.M.S.), the Research Corporation (M.G.H.), and the NIH (Grant GM49216 to J.K.B., predoctoral traineeship to S.O.K.).Additional details
- Eprint ID
- 88036
- DOI
- 10.1021/la980874n
- Resolver ID
- CaltechAUTHORS:20180719-155943234
- NSF
- Research Corporation
- NIH
- GM49216
- NIH Predoctoral Fellowship
- Created
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2018-07-19Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field