Feasibility of field-based light scattering spectroscopy
Abstract
Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible. A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 mm in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed.
Additional Information
© 2000 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper JBO-42014 received Sep. 15, 1999; revised manuscript received Feb. 8, 2000; accepted for publication Mar. 1, 2000. Valuable discussions with Vadim Backman are gratefully acknowledged, as well as help by Rajan Gurjar with various aspects of the experiment. This work was carried out at the MIT Laser Biomedical Research Center and was supported by National Institutes of Health Grant No. P41-RR02594 and a grant from Hamamatsu Corporation.Attached Files
Published - 138_1.pdf
Files
| Name | Size | Download all |
|---|---|---|
|
md5:923c0585797eb26403800d99e9ccd92e
|
224.3 kB | Preview Download |
Additional details
- Eprint ID
- 87768
- Resolver ID
- CaltechAUTHORS:20180711-143825869
- NIH
- P41-RR02594
- Hamamatsu Corporation
- Created
-
2018-07-16Created from EPrint's datestamp field
- Updated
-
2021-11-15Created from EPrint's last_modified field