Vibrational Imaging of Glucose Uptake Activity in Live Cells and Tissues by Stimulated Raman Scattering
Abstract
Glucose is a ubiquitous energy source for most living organisms. Its uptake activity closely reflects cellular metabolic demand in various physiopathological conditions. Extensive efforts have been made to specifically image glucose uptake, such as with positron emission tomography, magnetic resonance imaging, and fluorescence microscopy, but all have limitations. A new platform to visualize glucose uptake activity in live cells and tissues is presented that involves performing stimulated Raman scattering on a novel glucose analogue labeled with a small alkyne moiety. Cancer cells with differing metabolic activities can be distinguished. Heterogeneous uptake patterns are observed with clear cell-cell variations in tumor xenograft tissues, neuronal culture, and mouse brain tissues. By offering the distinct advantage of optical resolution but without the undesirable influence of fluorophores, this method will facilitate the study of energy demands of living systems with subcellular resolution.
Additional Information
© 2015 WILEY‐VCH. Received: March 18, 2015. Revised: April 26, 2015. Published online: July 16, 2015. We thank Y. Shin for providing hippocampal neurons and J. Hirtz for assistance on mouse brain tissues. W.M. acknowledges support from the NIH Director's New Innovator Award, ARO MURI W911NF-12-1-0594, and a Alfred P. Sloan Research Fellowship.Attached Files
Accepted Version - nihms732618.pdf
Supplemental Material - anie_201502543_sm_miscellaneous_information.pdf
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Additional details
- PMCID
- PMC4644272
- Eprint ID
- 86923
- DOI
- 10.1002/anie.201502543
- Resolver ID
- CaltechAUTHORS:20180608-124956778
- NIH
- W911NF-12-1-0594
- Army Research Office (ARO)
- Alfred P. Sloan Foundation
- Created
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2018-06-11Created from EPrint's datestamp field
- Updated
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2021-11-15Created from EPrint's last_modified field