Virtual phase conjugation based optical tomography for single-shot three-dimensional imaging
Abstract
We propose a virtual phase conjugation (VPC) based optical tomography (VPC-OT) for realizing single-shot optical tomographic imaging systems. Using a computer-based numerical beam propagation, the VPC combines pre-modulation and post-demodulation of the probe beam's wavefront, which provides an optical sectioning capability for resolving the depth coordinates. In VPC-OT, the physical optical microscope system and VPC are coupled using digital holography. Therefore, in contrast to conventional optical tomographic imaging (OTI) systems, this method does not require additional elements such as low-coherence light sources or confocal pinholes. It is challenging to obtain single-shot three-dimensional (3D) tomographic images using a conventional OTI system; however, this can be achieved using VPC-OT, which employs both digital holography and computer based numerical beam propagation. In addition, taking into account that VPC-OT is based on a complex amplitude detection using digital holography, this method allows us to simultaneously obtain quantitative phase contrast images. Using an objective lens with a numerical aperture (NA) of 0.8, we demonstrate a single-shot 3D imaging of frog blood cells with a depth resolution of 0.94 μm.
Additional Information
© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Received 30 Oct 2017; revised 7 Jan 2018; accepted 9 Jan 2018; published 5 Feb 2018. Funding: Japan Society for the Promotion of Science (JSPS) (JP17J01545).Attached Files
Published - oe-26-4-3779.pdf
Supplemental Material - Visualization_1.mov
Supplemental Material - Visualization_2.mov
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Additional details
- Eprint ID
- 85396
- Resolver ID
- CaltechAUTHORS:20180321-103708946
- JP17J01545
- Japan Society for the Promotion of Science (JSPS)
- Created
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2018-03-26Created from EPrint's datestamp field
- Updated
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2021-11-15Created from EPrint's last_modified field