Published March 15, 2018
| Accepted Version
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CRISPR Knockouts in Ciona Embryos
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage.
Additional Information
© 2018 Springer Nature Singapore Pte Ltd. First Online: 15 March 2018. Research in the laboratory of L.C. is supported by R01 awards HL108643 and GM096032 from the NIH/NHLBI and NIH/NIGMS respectively; and by grant 15CVD01 from the Leducq Foundation. A.S. is supported by R00 award HD084814 from the NIH/NICHD.Attached Files
Accepted Version - nihms-976213.pdf
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Additional details
- PMCID
- PMC6061950
- Eprint ID
- 85358
- Resolver ID
- CaltechAUTHORS:20180319-130924469
- HL108643
- NIH
- GM096032
- NIH
- 15CVD01
- Leducq Foundation
- HD084814
- NIH
- Created
-
2018-03-26Created from EPrint's datestamp field
- Updated
-
2021-11-15Created from EPrint's last_modified field
- Series Name
- Advances in Experimental Medicine and Biology
- Series Volume or Issue Number
- 1029