High-throughput ultraviolet photoacoustic microscopy with multifocal excitation

Creators: Imai, Toru; Shi, Junhui; Wong, Terence T. W.; Li, Lei; Zhu, Liren; Wang, Lihong V.

Abstract

Ultraviolet photoacoustic microscopy (UV-PAM) is a promising intraoperative tool for surgical margin assessment (SMA), one that can provide label-free histology-like images with high resolution. In this study, using a microlens array and a one-dimensional (1-D) array ultrasonic transducer, we developed a high-throughput multifocal UV-PAM (MF-UV-PAM). Our new system achieved a 1.6 ± 0.2 μm lateral resolution and produced images 40 times faster than the previously developed point-by-point scanning UV-PAM. MF-UV-PAM provided a readily comprehensible photoacoustic image of a mouse brain slice with specific absorption contrast in ∼16 min, highlighting cell nuclei. Individual cell nuclei could be clearly resolved, showing its practical potential for intraoperative SMA.

Additional Information

© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper 170796R received Dec. 8, 2017; accepted for publication Feb. 22, 2018; published online Mar. 15, 2018. The authors appreciate the close reading of the manuscript by Prof. James Ballard. This work was sponsored by National Institutes of Health (NIH) Grants R01 CA186567 (NIH Director's Transformative Research Award), U01 NS090579 (NIH BRAIN Initiative), U01 NS099717 (NIH BRAIN Initiative), and R01 EB016963, and supported in part by the March of Dimes Prematurity Research Center. L.V.W. has financial interests in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work.

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