Published February 6, 2018
| public
Book Section - Chapter
Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering
- Creators
- van Eldijk, Mark B.
- van Hest, Jan C. M.
Abstract
The incorporation of noncanonical amino acids has given protein chemists access to an expanded repertoire of amino acids. This methodology has significantly broadened the scope of protein engineering allowing introduction of amino acids with non-native functionalities, such as bioorthogonal reactive handles (azides and alkynes) and hydrophobic fluorinated side chains. Here, we describe the efficient residue-specific replacement of methionine by azidonorleucine in an engineered green fluorescent protein using a bacterial expression system to introduce a single reactive site for the strain-promoted azide-alkyne cycloaddition.
Additional Information
© 2018 Springer Science+Business Media, LLC. This work was financially supported by Netherlands Organisation for Scientific Research (Rubicon grant 680-50-1407 and Gravitation program 024.001.035). The plasmids encoding the mutant MetRS and GFP were a kind gift from Prof. D. A. Tirrell (California Institute of Technology).Additional details
- Eprint ID
- 84776
- Resolver ID
- CaltechAUTHORS:20180212-075402348
- 680-50-1407
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)
- 024.001.035
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)
- Created
-
2018-02-13Created from EPrint's datestamp field
- Updated
-
2021-11-15Created from EPrint's last_modified field
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 1728