Published March 31, 2013
| Accepted Version + Supplemental Material
Journal Article
Open
The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome
Chicago
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Abstract
Decoding of the AUA isoleucine codon in bacteria and archaea requires modification of a C in the anticodon wobble position of the isoleucine tRNA. Here, we report the crystal structure of the archaeal tRNA_2^(Ile), which contains the modification agmatidine in its anticodon, in complex with the AUA codon on the 70S ribosome. The structure illustrates how agmatidine confers codon specificity for AUA over AUG.
Additional Information
© 2013 Macmillan Publishers Limited, part of Springer Nature. Received: 14 December 2012; Accepted: 15 February 2013; Published online: 31 March 2013. We thank A. McCarthy and S. Brockhauser at European Synchrotron Radiation Facility ID14.4 for facilitating data collection. This work was supported by the Medical Research Council, UK, grant U105184332 (V.R.), the Wellcome Trust (V.R.), the Agouron Institute (V.R.) and the Louis-Jeantet Foundation (V.R.), and grant GM17151 from the US National Institutes of Health (U.L.R.). Support was also received from the Gates-Cambridge scholarship and Peterhouse (R.V.) and from Boehringer Ingelheim (C.N.). Accession codes: Coordinates and structural factors have been deposited in the Protein Data Bank, with codes 3ZN7, 3ZN9, 3ZND and 3ZNE. Author Contributions:D.M. and C.K. purified tRNA substrates; R.M.V. and C.N. grew crystals, collected data, built and refined the structure, and analyzed the model; R.M.V., C.K., U.L.R. and V.R. wrote and edited the manuscript. The authors declare no competing financial interests.Attached Files
Accepted Version - emss-52003.pdf
Supplemental Material - nsmb.2545-S1.pdf
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Additional details
- PMCID
- PMC3672977
- Eprint ID
- 84349
- DOI
- 10.1038/nsmb.2545
- Resolver ID
- CaltechAUTHORS:20180116-155912862
- Medical Research Council (UK)
- U105184332
- Wellcome Trust
- Agouron Institute
- Louis-Jeantet Foundation
- NIH
- GM17151
- Gates-Cambridge Scholarship
- Peterhouse
- Boehringer Ingelheim
- Created
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2018-01-17Created from EPrint's datestamp field
- Updated
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2023-02-28Created from EPrint's last_modified field