Population regulation in microbial consortia using dual feedback control
Abstract
An ongoing area of study in synthetic biology has been the design and construction of synthetic circuits that maintain homeostasis at the population level. Here, we are interested in designing a synthetic control circuit that regulates the total cell population and the relative ratio between cell strains in a culture containing two different cell strains. We have developed a dual feedback control strategy that uses two separate control loops to achieve the two functions respectively. By combining both of these control loops, we have created a population regulation circuit where both the total population size and relative cell type ratio can be set by reference signals. The dynamics of the regulation circuit show robustness and adaptation to perturbations in cell growth rate and changes in cell numbers. The control architecture is general and could apply to any organism for which synthetic biology tools for quorum sensing, comparison between outputs, and growth control are available.
Additional Information
The copyright holder for this preprint is the author/funder. bioRxiv preprint first posted online Mar. 24, 2017. The authors would like to thank Reed McCardell and Victoria Hsiao for their insightful discussion. The authors X. R., A. B., and A. S. are partially supported by the Air Force Office of Scientific Research, grant number FA9550-14-1-0060. The project depicted is also sponsored by the Defense Advanced Research Projects Agency (Agreement HR0011-17-2-0008). The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.Attached Files
Submitted - 120253.full.pdf
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Additional details
- Eprint ID
- 81795
- Resolver ID
- CaltechAUTHORS:20170925-083417843
- FA9550-14-1-0060
- Air Force Office of Scientific Research (AFOSR)
- HR0011-17-2-0008
- Defense Advanced Research Projects Agency (DARPA)
- Created
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2017-09-25Created from EPrint's datestamp field
- Updated
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2023-06-01Created from EPrint's last_modified field