Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery
Abstract
We are utilizing the folate receptor for the intracellular delivery of DNA. In this study, a folate-poly-L-lysine (FPLL) conjugate was synthesized and equilibrated with plasmid DNA encoding the firefly luciferase gene. The FPLL-DN_A complexes were added to KB cells treated with chloroquine. Luciferase activity of cells incubated with FPLL-DNA was 6-fold higher than of cells exposed to poly-L-lysine. (PLL)-DNA. The addition of free folic acid competitively inhibited the enhancement of gene expression. Removal of chloroquine from the media significantly inhibited transfection efficiency of FPLL-DNA complexes. We conclude that FPLL-DNA complexes are delivered into KB cells via folate receptor-mediated endocytosis and likely follow a lysosomal pathway into the cytoplasm.
Additional Information
© 1995 American Chemical Society. Received June 15, 1995. This work was funded in part by a USPHS Training Grant No. GM08346, Nexstar, Inc., and the Biological Imaging Center. We thank Dr. Rex Moats and Dr. Wilton Vannier for helpful discussions.Additional details
- Eprint ID
- 81128
- DOI
- 10.1021/bc00035a002
- Resolver ID
- CaltechAUTHORS:20170905-095408869
- GM08346
- NIH Predoctoral Fellowship
- Nexstar, Inc.
- Created
-
2017-09-05Created from EPrint's datestamp field
- Updated
-
2021-11-15Created from EPrint's last_modified field