Published March 2, 2009
| Accepted Version
Journal Article
Open
Fluorescence Detection of a Protein-Bound 2Fe2S Cluster
Abstract
A fluorescent biosensor is described for 2Fe2S clusters that is composed of green fluorescent protein (GFP) fused to glutaredoxin 2 (Grx2), as illustrated here. 2Fe2S detection is based on the reduction of GFP fluorescence upon the 2Fe2S-induced dimerization of GFP-Grx2. This assay is sufficiently sensitive to detect submicromolar changes in 2Fe2S levels, thus making it suitable for high-throughput measurements of metallocluster degradation and synthesis reactions.
Additional Information
© 2009 Wiley. Issue online: 24 Feb 2009 Version of Record online: 30 Jan 2009 Manuscript Received: 12 Nov 2008 This work was supported by the Robert A. Welch Foundation (C-1614; to J.J.S.), Arnold and Mabel Beckman Foundation (to C.D.S.), and a National Institutes of Health Biotechnology fellowship (5F32M078901; to K.G.H.).Attached Files
Accepted Version - nihms149605.pdf
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Additional details
- PMCID
- PMC3897124
- Eprint ID
- 79343
- DOI
- 10.1002/cbic.200800747
- Resolver ID
- CaltechAUTHORS:20170725-132756192
- C-1614
- Robert A. Welch Foundation
- Arnold and Mabel Beckman Foundation
- 5F32M078901
- NIH Predoctoral Fellowship
- Created
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2017-07-25Created from EPrint's datestamp field
- Updated
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2021-11-15Created from EPrint's last_modified field