Theory of long binding events in single-molecule–controlled rotation experiments on F_1-ATPase
- Creators
- Volkán-Kacsó, Sándor
- Marcus, Rudolph A.
Abstract
The theory of elastic group transfer for the binding and release rate constants for nucleotides in F_1-ATPase as a function of the rotor angle is further extended in several respects. (i) A method is described for predicting the experimentally observed lifetime distribution of long binding events in the controlled rotation experiments by taking into account the hydrolysis and synthesis reactions occurring during these events. (ii) A method is also given for treating the long binding events in the experiments and obtaining the rate constants for the hydrolysis and synthesis reactions occurring during these events. (iii) The theory in the previous paper is given in a symmetric form, an extension that simplifies the application of the theory to experiments. It also includes a theory-based correction of the reported "on" and "off" rates by calculating the missed events. A near symmetry of the data about the angle of −40° and a "turnover" in the binding rate data vs. rotor angle for angles greater than ∼∼40° is also discussed.
Additional Information
© 2017 National Academy of Sciences. Contributed by Rudolph A. Marcus, May 17, 2017 (sent for review April 10, 2017; reviewed by Attila Szabo and Arieh Warshel). Published online before print June 26, 2017. We thank Drs. Hiroyuki Noji and Kengo Adachi for helpful discussions and comments. This work was supported by the Office of the Naval Research, the Army Research Office, and the James W. Glanville Foundation. Author contributions: S.V.-K. and R.A.M. designed research; S.V.-K. and R.A.M. performed research; S.V.-K. contributed new reagents/analytic tools; S.V.-K. and R.A.M. analyzed data; and S.V.-K. and R.A.M. wrote the paper. The authors declare no conflict of interest. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1705960114/-/DCSupplemental.Attached Files
Published - PNAS-2017-Volkán-Kacsó-7272-7.pdf
Supplemental Material - pnas.1705960114.sapp.pdf
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Additional details
- PMCID
- PMC5514755
- Eprint ID
- 78642
- DOI
- 10.1073/pnas.1705960114
- Resolver ID
- CaltechAUTHORS:20170628-102612597
- Office of Naval Research (ONR)
- Army Research Office (ARO)
- James W. Glanville Foundation
- Created
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2017-06-28Created from EPrint's datestamp field
- Updated
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2022-03-24Created from EPrint's last_modified field