Published June 4, 2014
| public
Book Section - Chapter
Extraction and Measurement of NAD(P)+ and NAD(P)H
- Others:
- Filloux, Alain
- Ramos, Juan-Luis
Chicago
Abstract
Nicotinamide adenine dinucleotides are critical redox-active substrates for countless catabolic and anabolic reactions. Ratios of NAD+ to NADH and NADP+ to NADPH are therefore considered key indicators of the overall intracellular redox potential and metabolic state. These ratios can be measured in bulk conditions using a highly sensitive enzyme cycling-based colorimetric assay (detection limit at or below 0.05 μM or 1 pmol) following a simple extraction procedure involving solutions of acid and base. Special considerations are necessary to avoid measurement artifacts caused by the presence of endogenous redox-active metabolites, such as phenazines made by diverse Pseudomonas species (see Chapter 25).
Additional Information
Ⓒ 2014 Springer Science+Business Media New York.Additional details
- Eprint ID
- 77593
- Resolver ID
- CaltechAUTHORS:20170518-165224584
- Created
-
2017-05-19Created from EPrint's datestamp field
- Updated
-
2021-11-15Created from EPrint's last_modified field
- Caltech groups
- Division of Geological and Planetary Sciences
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 1149