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Published November 27, 2002 | public
Journal Article Metadata-only

Whole-Genome Analysis of Dorsal-Ventral Patterning in the Drosophila Embryo

Stathopoulos, Angelike ORCID icon
Van Drenth, Madeleine
Erives, Albert
Markstein, Michele
Levine, Michael

Abstract

The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tissue-specific form of the NF-Y transcription complex is essential for the activation of gene expression in the mesoderm. Tissue-specific enhancers were identified for new Dorsal target genes, and bioinformatics methods identified conserved cis-regulatory elements for coordinately regulated genes that respond to similar thresholds of the Dorsal gradient. The new Dorsal target genes and enhancers represent one of the most extensive gene networks known for any developmental process.

Additional Information

© 2002 Cell Press. Published by Elsevier Inc. Received 30 May 2002, Revised 3 October 2002. We thank M. Blanchette, A. Hochheimer, E. Laborier, D. Rio, and R. Tjian for advice and technical assistance with the microarray experiments, and L. Mirels and R. Zinzen for critically reading the manuscript. We also thank R. Zinzen for help with Figure 5M and K. Senger for sharing SELEX data. P. Markstein independently identified the CACATGT motif using a software package, Mermaid, that is under development. We are grateful to H. Ashe for the Kr-FSF expression vector and probes and Y. Nibu for providing ESTs. This work was funded by a grant from the NIH (GM46638) to M.L. A.S. is supported by a postdoctoral fellowship from the NIH (GM20352). Accession Numbers: The array data have been deposited in the Gene Expression Omnibus at NCBI (GEO: http://www.ncbi.nlm.nih.gov/geo) as series GSE86: GSM2452 (Toll^(rm9/rm10) versus Toll^(10b)), GSM2453 (pipe versus Toll^(10b)), and GSM2454 (pipe versus Toll^(rm9/rm10)). The vnd, Mes3, and Neu4 embryonic enhancer DNA sequences have been deposited into GenBank with accession numbers BK000635, BK000634, and BK000636, respectively.

Additional details

Identifiers Funding Dates
Created:
August 19, 2023
Modified:
October 25, 2023