Published May 10, 2006
| Accepted Version + Supplemental Material
Journal Article
Open
Enantioselective α-Hydroxylation of 2-Arylacetic Acid Derivatives and Buspirone Catalyzed by Engineered Cytochrome P450 BM-3
Chicago
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Abstract
Here we report that an engineered microbial cytochrome P450 BM-3 (CYP102A subfamily) efficiently catalyzes the α-hydroxylation of phenylacetic acid esters. This P450 BM-3 variant also produces the authentic human metabolite of buspirone, R-6-hydroxybuspirone, with 99.5% ee.
Additional Information
© 2006 American Chemical Society. Received February 22, 2006. Publication Date (Web): April 13, 2006. Financial support from the National Science Foundation (BES9981770), NIH Grant R01 GM068664-01, NSF Graduate Research Fellowship program (L.H.), and the Swedish Foundation for Strategic Research (A.K. and J.E.B.) is gratefully acknowledged. Special thanks to N. Dalleska and M. Chen for assistance with the GC, P. Meinhold for cloning the 9-10A-F87A variant, and R. Patel, S. Kiang, and J. Depue (Bristol-Myers Squibb) for assistance with the buspirone analytics.Attached Files
Accepted Version - nihms63675.pdf
Supplemental Material - ja061261xsi20060222_015822.pdf
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ja061261xsi20060222_015822.pdf
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Additional details
- PMCID
- PMC2551755
- Eprint ID
- 76846
- DOI
- 10.1021/ja061261x
- Resolver ID
- CaltechAUTHORS:20170424-103335679
- NSF
- BES-9981770
- NIH
- R01 GM068664-01
- NSF Graduate Research Fellowship
- Swedish Foundation for Strategic Research
- Created
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2017-04-24Created from EPrint's datestamp field
- Updated
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2021-11-15Created from EPrint's last_modified field