Gold Electrodes Wired for Coupling with the Deeply Buried Active Site of Arthrobacter globiformis Amine Oxidase

Creators: Hess, Corinna R.; Juda, Gregory A.; Dooley, David M.; Amii, Ricky N.; Hill, Michael G.; Winkler, Jay R.; Gray, Harry B.

Abstract

Diethylaniline-terminated oligo(phenyl-ethynyl)-thiol (DEA-OPE-SH) wires on Au-bead electrodes facilitate electron tunneling to and from the deeply buried topaquinone (TPQ) cofactor in Arthrobacter globiformis amine oxidase (AGAO). Reversible cyclic voltammograms were observed when AGAO was adsorbed onto this DEA-OPE-SAu surface: the 2e^-/2H^+ reduction potential is −140 mV versus SCE.

Additional Information

© 2003 American Chemical Society. Received 1 December 2002. Published online 22 May 2003. Published in print 1 June 2003. This work is dedicated to the memory of Eraldo Antonini (per il ventesimo anniversario della sua morte, 19 Marzo 2003), a giant in metallobiochemistry. We thank R. Tanimura and K. Niki for assistance in the preparation of Au-bead electrodes and for helpful discussions. This work was supported by NIH (C.R.H., J.R.W., H.B.G., G.A.J., D.M.D.) and the David and Lucille Packard Foundation Initiative for Interdisciplinary Research (R.N.A., M.G.H.).

Attached Files

Supplemental Material - ja029538qsi20030313_023427.pdf

Files

ja029538qsi20030313_023427.pdf

Files (136.7 kB)

Name	Size	Download all
ja029538qsi20030313_023427.pdf md5:e663581f4c88eb04a348098a7236f512	136.7 kB	Preview Download

Additional details

	All versions	This version
Views	0	0
Downloads	0	0
Data volume	0 Bytes	0 Bytes