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Published December 13, 2007 | Accepted Version + Supplemental Material
Journal Article Open

Stage-specific differences in the requirements for germline stem cell maintenance in the Drosophila ovary

Abstract

In this study, we uncover a role for microRNAs (miRNAs) in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam miRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-β pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations and suggest that there may be a Dicer-2-dependent GSC maintenance mechanism during development that is lost in later life.

Additional Information

© 2007 Elsevier. Received 24 April 2007, Revised 11 September 2007, Accepted 21 November 2007, Available online 12 December 2007. We thank Drs. A. Spradling, D. Pan, B. Calvi, S. Cohen, T.T. Su, T. Orr-Weaver, T. Xie, J. Tamkun, and R. Carthew for suggestions, flies, and reagents, Christian Nelson, Louise von Stechow, and Andriy Yatsenko for help with experiments, and Dr. Bradford Stadler for help with QPCR analysis. This work was supported by AHA fellowships for H.R.S. and S.H.R. and MOD and NIH grants and the Tietze Fellowship for H.R-B.

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Accepted Version - nihms36251.pdf

Supplemental Material - 1-s2.0-S1934590907002809-mmc1.pdf

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