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Published April 2007 | Supplemental Material
Journal Article Open

The Transcription Factor WIN1/SHN1 Regulates Cutin Biosynthesis in Arabidopsis thaliana

Abstract

he composition and permeability of the cuticle has a large influence on its ability to protect the plant against various forms of biotic and abiotic stress. WAX INDUCER1 (WIN1) and related transcription factors have recently been shown to trigger wax production, enhance drought tolerance, and modulate cuticular permeability when overexpressed in Arabidopsis thaliana. We found that WIN1 influences the composition of cutin, a polyester that forms the backbone of the cuticle. WIN1 overexpression induces compositional changes and an overall increase in cutin production in vegetative and reproductive organs, while its downregulation has the opposite effect. Changes in cutin composition are preceded by the rapid and coordinated induction of several genes known or likely to be involved in cutin biosynthesis. This transcriptional response is followed after a delay by the induction of genes associated with wax biosynthesis, suggesting that the regulation of cutin and wax production by WIN1 is a two-step process. We demonstrate that at least one of the cutin pathway genes, which encodes long-chain acyl-CoA synthetase LACS2, is likely to be directly targeted by WIN1. Overall, our results suggest that WIN1 modulates cuticle permeability in Arabidopsis by regulating genes encoding cutin pathway enzymes.

Additional Information

© 2007 The American Society of Plant Biologists. Received September 4, 2006; revised March 14, 2007; accepted April 3, 2007; published April 20, 2007. We thank Toshiro Ito for providing the pGTI0242 vector, Christiane Nawrath for her assistance with mass spectral analysis, Naveed Aziz for his technical support with microarray hybridizations and data analysis, and Uzma Urooj for her technical assistance with this project. This work was supported by Grant P20283 from the Biotechnology and Biological Sciences Research Council (UK). The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Pierre Broun (pbroun@york.ac.uk).

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Supplemental Material - Supplemental_Figure_5.doc

Supplemental Material - Supplemental_Figure_6.doc

Supplemental Material - Supplemental_Table_3.doc

Supplemental Material - Supplemental_table_1_revised.xls

Supplemental Material - Supplemental_table_2.xls

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August 19, 2023
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