Direct Assessment of MHC Class I Binding by Seven Ly49 Inhibitory NK Cell Receptors
Abstract
Mouse NK cells express at least seven inhibitory Ly49 receptors. Here we employ a semiquantitative cell–cell adhesion assay as well as class I/peptide tetramers to provide a comprehensive analysis of specificities of Ly49 receptors for class I MHC molecules in eight MHC haplotypes. Different Ly49 receptors exhibited diverse binding properties. The degree of class I binding was related to the extent of functional inhibition. The tetramer studies demonstrated that neither glycosylation nor coreceptors were necessary for class I binding to Ly49 receptors and uncovered peptide-specific recognition by a Ly49 receptor. The results provide a foundation for interpreting and integrating many existing functional studies as well as for designing tests of NK cell development and self-tolerance.
Additional Information
© 1999 Cell Press. Received 8 March 1999, Revised 20 May 1999. We thank Peter Snow for production of the recombinant Dd from baculovirus, Vinay Kumar and Porunelloor Mathew for the cDNA library, Werner Held for Ly49A transgenic mice, Chern-sing Goh and Ann Lazar for technical assistance, and Russell Vance for critical reading of the manuscript. T. H. was a recipient of a Research Fellowship from the Deutsche Forschungsgemeinschaft, C. W. M. is the recipient of a Cancer Research Institute/Chase Manhattan Bank fellowship, and D. H. B. is a recipient of a Research Fellowship from the Howard Hughes Medical Institute. F. A. L. was supported by the Institut Pasteur. This work was supported by National Institutes of Health grant RO-1AI35021 to D. H. R.Additional details
- Eprint ID
- 75755
- DOI
- 10.1016/S1074-7613(00)80082-5
- Resolver ID
- CaltechAUTHORS:20170405-154817252
- Deutsche Forschungsgemeinschaft (DFG)
- Cancer Research Institute
- Chase Manhattan Bank
- Howard Hughes Medical Institute (HHMI)
- Institut Pasteur
- RO-1AI35021
- NIH
- Created
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2017-04-05Created from EPrint's datestamp field
- Updated
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2021-11-15Created from EPrint's last_modified field