Published May 14, 2008
| Published
Journal Article
Open
LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
Abstract
New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging.
Additional Information
© 2008 Albeanu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received September 21, 2007; Accepted April 3, 2008; Published May 14, 2008. Support was only from start up funds provided by Harvard University to the senior authors. These funds have no restrictions and the University does not direct the research in any way. Author Contributions: Conceived and designed the experiments: VM MM DA ES. Performed the experiments: DA ES. Analyzed the data: DA TS. Contributed reagents/materials/analysis tools: VM MM. Wrote the paper: VM MM DA ES TS. The authors have declared that no competing interests exist.Attached Files
Published - journal.pone.0002146.PDF
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journal.pone.0002146.PDF
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Additional details
- PMCID
- PMC2361193
- Eprint ID
- 75731
- Resolver ID
- CaltechAUTHORS:20170405-101751665
- Harvard University
- Created
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2017-04-05Created from EPrint's datestamp field
- Updated
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2021-11-15Created from EPrint's last_modified field