Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division
Abstract
The mechanism by which bacteria divide is not well understood. Cell division is mediated by filaments of FtsZ and FtsA (FtsAZ) that recruit septal peptidoglycan-synthesizing enzymes to the division site. To understand how these components coordinate to divide cells, we visualized their movements relative to the dynamics of cell wall synthesis during cytokinesis. We found that the division septum was built at discrete sites that moved around the division plane. FtsAZ filaments treadmilled circumferentially around the division ring and drove the motions of the peptidoglycan-synthesizing enzymes. The FtsZ treadmilling rate controlled both the rate of peptidoglycan synthesis and cell division. Thus, FtsZ treadmilling guides the progressive insertion of new cell wall by building increasingly smaller concentric rings of peptidoglycan to divide the cell.
Additional Information
© 2017 American Association for the Advancement of Science. 26 September 2016; accepted 20 January 2017. We thank F. Gueiros-Filho, R. Losick, M. Erb, and P. Levin for strains; J. Xiao for discussions; L. Lavis for Janelia Fluor dyes; B. Murphy and E. Pasciak for FDAA synthesis help; F. Gueiros-Filho, R. Daniel, and J. Errington for antibodies; R. Losick, B. LaSarre, and D. Kearns for comments. This work was supported by NIH grants GM113172 to M.S.V. and Y.V.B., GM51986 to Y.V.B., and DP2AI117923-01 to E.C.G.; a Newcastle University Research Fellowship and Royal Society Research Grant RG150475 to S.H.; a Science Without Borders Research Fellowship to A.W.B.-F.; a European Research Council Advanced Grant (SynDiv 669598) to C.D.; and an NSF Graduate Research Fellowship Program (DGE1144152) to G.R.S. SIM was performed in the Indiana University Light Microscopy Imaging Center supported by S10RR028697-01. E.K., Y.V.B., and M.S.V. are inventors on U.S. Patent Application 14/395,815 (PCT Patent Application PCT/US2013/037504), submitted by the Indiana University Research and Technology Corporation, that covers the use of FDAAs for labeling of bacterial cell walls. Data are available in the text and supplementary materials. Movies S1 to S10 are also available at http://garnerlab.fas.harvard.edu/FtsZ/. Code is available at https://bitbucket.org/garnerlab/bisson-2016.Attached Files
Supplemental Material - aak9973_Bisson-Filho_SM.pdf
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Additional details
- Eprint ID
- 75573
- Resolver ID
- CaltechAUTHORS:20170331-100705359
- GM113172
- NIH
- GM51986
- NIH
- DP2AI117923-01
- NIH
- Newcastle University
- RG150475
- Royal Society
- Science Without Borders
- 669598 SynDiv
- European Research Council (ERC)
- DGE-1144152
- NSF Graduate Research Fellowship
- S10RR028697-01
- NIH
- Created
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2017-03-31Created from EPrint's datestamp field
- Updated
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2021-11-15Created from EPrint's last_modified field