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Published August 1978 | public
Journal Article

Heteroduplex Analysis of the Nonhomology Region between Moloney MuLV and the Dual Host Range Derivative HIX Virus

Abstract

The dual host range virus HIX has been previously characterized as an envelope gene recombinant between Moloney murine leukemia virus (Mo-MuLV) and an unidentified xenotropic murine leukemia virus. Using long reverse transcripts of Mo-MuLV, a region of nonhomology has been mapped by electron microscopic analysis of heteroduplexes formed with HIX 35S virion RNA. In this nonhomology region, the Mo-MuLV cDNA strand measured approximately 900 nucleotides, mapping between 1.6 and 2.5 kilobases from the 3′ end. In a previous study, hybridization of Mo-MuLV 21S RNA with Mo-MuLV cDNA resulted in the formation of different heteroduplex structures diagnostic of a noncontiguously coded leader sequence at the 5′ end of the 21S RNA. Following hybridization of poly(A)+ HIX 21S RNA with 8.2 kb Mo-MuLV cDNA, analogous heteroduplex structures were observed exhibiting the Mo-MuLV:HIX substitution loop in the DNA:RNA segment of the molecules. This analysis permitted more precise mapping of the nonhomology region with respect to the splice point in the 21S presumptive glycoprotein mRNA. The mapping of this nonhomology region in HIX virus provides an internal visual marker for the 3′ end of the genome which may prove useful in future analyses of other deletion or substitution derivatives of Mo-MuLV.

Additional Information

© 1978 by MIT. Received 18 April 1978, Revised 23 May 1978. D.J.D. and E.R. particularly wish to thank the following individuals for stimulating discussions and encouragement: Poul Andersson, Arnold Berk, Peter Besmer, Douglas Faller and Owen Witte. D.J.D. gratefully acknowledges a Whitaker Health Sciences Fund Fellowship. E.R. is supported by a postdoctoral fellowship from the Jane Coffin Childs Memorial Fund for Cancer Research. D.B. is a Research Professor of the American Cancer Society. P.A.S. gratefully acknowledges a Career Development Award from the American Cancer Society. This work was supported by a Cancer Center Core Grant from the NIH. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Additional details

Created:
August 19, 2023
Modified:
October 24, 2023