Three-Dimensional Quantitative Structure−Activity Relationship of Nucleosides Acting at the A_3 Adenosine Receptor: Analysis of Binding and Relative Efficacy
- Creators
- Kim, Soo-Kyung
- Jacobson, Kenneth A.
Abstract
The binding affinity and relative maximal efficacy of human A_3 adenosine receptor (AR) agonists were each subjected to ligand-based three-dimensional quantitative structure−activity relationship analysis. Comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) used as training sets a series of 91 structurally diverse adenosine analogues with modifications at the N^6 and C2 positions of the adenine ring and at the 3', 4', and 5' positions of the ribose moiety. The CoMFA and CoMSIA models yielded significant cross-validated q^2 values of 0.53 (r^2 = 0.92) and 0.59 (r^2 = 0.92), respectively, and were further validated by an external test set (25 adenosine derivatives), resulting in the best predictive r^2 values of 0.84 and 0.70 in each model. Both the CoMFA and the CoMSIA maps for steric or hydrophobic, electrostatic, and hydrogen-bonding interactions well reflected the nature of the putative binding site previously obtained by molecular docking. A conformationally restricted bulky group at the N^6 or C2 position of the adenine ring and a hydrophilic and/or H-bonding group at the 5' position were predicted to increase A_3AR binding affinity. A small hydrophobic group at N^6 promotes receptor activation. A hydrophilic and hydrogen-bonding moiety at the 5' position appears to contribute to the receptor activation process, associated with the conformational change of transmembrane domains 5, 6, and 7. The 3D-CoMFA/CoMSIA model correlates well with previous receptor-docking results, current data of A_3AR agonists, and the successful conversion of the A_3AR agonist into antagonists by substitution (at N^6) or conformational constraint (at 5'-N-methyluronamide).
Additional Information
© 2007 American Chemical Society. Received 8 November 2006. Published online 6 March 2007. Published in print 1 May 2007. This research was supported by the Intramural Research Program of the NIH, National Institute of Diabetes and Digestive and Kidney Diseases. We thank Dr. Andrei A. Ivanov, Krishnan K. Palaniappan, and Dr. Zhan-Guo Gao (NIDDK); Prof. Serge Van Calenbergh (University of Ghent, Belgium); and Prof. Lak Shin Jeong (EWHA Womens University, Seoul, Korea) for helpful discussions.Attached Files
Supplemental Material - ci600501z.pdf
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Additional details
- Eprint ID
- 73934
- Resolver ID
- CaltechAUTHORS:20170201-111306968
- NIH Intramural Research Program
- National Institute of Diabetes and Digestive and Kidney Diseases
- Created
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2017-02-01Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field