Cyclodextrin-Modified Polyethylenimine Polymers for Gene Delivery
Abstract
Linear and branched poly(ethylenimines), lPEI and bPEI, respectively, grafted with β-cyclodextrin are prepared to give CD−lPEI and CD−bPEI, respectively, and are investigated as in vitro and in vivo nonviral gene delivery agents. The in vitro toxicity and transfection efficiency are sensitive to the level of cyclodextrin grafting. The cyclodextrin-containing polycations, when combined with adamantane−poly(ethylene glycol) (AD−PEG) conjugates, form particles that are stable at physiological salt concentrations. PEGylated CD−lPEI-based particles give in vitro gene expression equal to or greater than lPEI as measured by the percentage of EGFP expressing cells. Tail vein injections into mice of 120 μg of plasmid DNA formulated with CD−lPEI and AD−PEG do not reveal observable toxicities, and both nucleic acid accumulation and expression are observed in liver.
Additional Information
© 2004 American Chemical Society. Received 3 May 2004; Published online 29 June 2004; Published in print 1 July 2004. J.D.H. acknowledges the Whitaker Foundation for a doctoral fellowship.Additional details
- Eprint ID
- 73707
- DOI
- 10.1021/bc049891g
- Resolver ID
- CaltechAUTHORS:20170125-093852180
- Whitaker Foundation
- Created
-
2017-01-25Created from EPrint's datestamp field
- Updated
-
2021-11-11Created from EPrint's last_modified field