Published April 25, 1991
| Published
Journal Article
Open
Rapid isolation of long cDNA clones from existing libraries
Chicago
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Abstract
Obtaining full-length or even near full-length cDNA clones has been a time-consuming and labor-intensive step in the analysis of cloned genes. Recent methods which use PCR as a preparative tool for cloning (1,2) have made this step considerably more rapid, but may introduce sequence errors in the resulting clones due to numerous sequential rounds of in vitro replication. We describe a method for identifying long cDNA clones from existing cDNA libraries using PCR purely as a diagnostic tool.
Additional Information
© 1991 Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. Submitted February 5, 1991. We thank M. Strathmann, K.Vijay Raghavan, and T.Wilke for constructive discussions and C.Mayeda and M.Whitney for excellent technical assistance. B.A.H. was supported in part by a USPHS Predoctoral National Research Service Award (T32 GM07616). This work was supported by a scholar's grant from the Lucille P.Markey Charitable Trust (M.J.P.) and USPHS Program Project Grant GM40499 (E.M.M.).Attached Files
Published - Nucl._Acids_Res.-1991-Hamilton-1951-2.pdf
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Nucl._Acids_Res.-1991-Hamilton-1951-2.pdf
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Additional details
- PMCID
- PMC328137
- Eprint ID
- 72819
- Resolver ID
- CaltechAUTHORS:20161214-120431504
- NIH Predoctoral Fellowship
- T32 GM07616
- Lucille P. Markey Charitable Trust
- NIH
- GM40499
- Created
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2016-12-14Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field