Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published November 2007 | Supplemental Material
Journal Article Open

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

Abstract

To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.

Additional Information

© 2007 American Society of Plant Biologists. Received June 21, 2007. Accepted September 14, 2007. Published September 28, 2007. This work was supported by the National Institutes of Health (grant no. GM45697 to E.M.M.), the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology, the Conselho Nacional de Desenvolvimento Científico e Tecnológico (grant nos. 307219/2004-6, 400767/2004-0, and 475666/2004-6 to M.A.-F. and fellowship to A.B.), the Fundacxão Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (grant no. E-26/171.332/2004 to M.A.-F. and fellowship to A.B.), the International Foundation for Science (grant no. C/3962-1 to M.A.-F.), the International Basic Sciences Programme (grant no. IBSP/UNESCO-3–BR-28 to M.A.-F.), and Aventis Crop Sciences (fellowship to M.A.-F.). We thank Arnavaz Garda and Beatriz Dias for technical assistance.

Attached Files

Supplemental Material - 104422Supplemental__Figure_1_Alves-Ferreira_et_al.pdf

Supplemental Material - 104422Supplemental_figure_2.pdf

Supplemental Material - 104422Supplemental_figure_3_Alves-Ferreira_el_al.pdf

Supplemental Material - 104422Supplemental_figure_4_Alves-Ferreira_et_al.pdf

Supplemental Material - 104422Supplemental_figure_5_Alves-Ferreira_et_al.pdf

Supplemental Material - 104422supplegends.pdf

Supplemental Material - 104422supptbl1.xls

Supplemental Material - 104422supptbl2.xls

Supplemental Material - 104422supptbl3.xls

Supplemental Material - 104422supptbl4.xls

Supplemental Material - 104422supptbl5.xls

Supplemental Material - 104422supptbl6.xls

Supplemental Material - 104422supptbl7.xls

Files

104422Supplemental_figure_4_Alves-Ferreira_et_al.pdf
Files (4.2 MB)
Name Size Download all
md5:5ba7ad3bf347c1b8ada05cd7694fb2d9
1.1 MB Download
md5:46190167dfde5790e1f6532f20d4d43c
40.4 kB Download
md5:14b1d489baf8ce305bce9c4c162553d2
83.1 kB Preview Download
md5:cbd0b7c6a339c1e1dfd0e215b6c2b0c0
43.6 kB Preview Download
md5:4a4cbceabdc026fcb3b8c016d23db969
18.8 kB Preview Download
md5:b459374a7788ef05a806c48ea01c9d37
18.9 kB Download
md5:bedba62c353e96837701a973cbadafd0
558.6 kB Download
md5:12c7b94ed3a23db994cd4d38a501c5b0
16.9 kB Download
md5:edd8035db89e2fc13f360d151869af00
14.8 kB Download
md5:977055ce166cef4d2478ed9e4fc05e1a
2.2 MB Download
md5:3a7dcf1c7f4a5563ed1e20985047dcb3
43.0 kB Preview Download
md5:bf3df37e581e9adefa1358ff86c726eb
49.0 kB Preview Download
md5:ee17a7fddd3bdd5e44f48f1f73d187cd
42.1 kB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 23, 2023