Published June 2015
| Accepted Version + Supplemental Material
Journal Article
Open
Quantitative assessments of glycolysis from single cells
Abstract
The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18]fluoro-D-glucose (^(18)F-FDG). We report on an integrated microfluidics-chip/beta particle imaging system for in vitro ^(18)F-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.
Additional Information
© 2015 World Scientific Publishing Co Pte Ltd. Received: 14 March 2015; Accepted: 5 May 2015; Published: 25 June 2015. This work was supported by the National Cancer Institute grant 5U54 CA151819 (JRH PI), the Ben and Catherine Ivy Foundation, the Jean Perkins Foundation, the NCI In Vivo Cellular and Molecular Imaging Center (ICMIC) and the Phelps Family Foundation. A.D. was supported in part by the UCLA Scholars in Oncologic Molecular Imaging program, NIH grant R25T CA098010. Y.S.S. acknowledges the support from the Korean-American Scientists and Engineers Association (KSEA). Author Contributions: Y.S.S. and J.K. developed microfluidic device, designed and performed tests. D.J., W.X.M., and L.T. prepared biological samples for tests. A.A.D. and A.F.C. developed the Betabox camera and software. D.A.N. and M.E.P. provided detailed guidelines and discussion for the experimental design and interpretation of the results. Y.S.S., J.K., and J.R.H. wrote the manuscript. J.R.H. and Y.S.S. directed the research. Competing Interests Statement: M.E.P., A.F.C., and J.R.H. are founders and stockholders in Sofie Bio-sciences, Inc., which is seeking to commercialize certain aspects of the Betabox technology.Attached Files
Accepted Version - nihms-704120.pdf
Supplemental Material - NIHMS704120-supplement-01.pdf
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NIHMS704120-supplement-01.pdf
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Additional details
- PMCID
- PMC4728151
- Eprint ID
- 71603
- Resolver ID
- CaltechAUTHORS:20161031-083900031
- 5U54 CA151819
- NIH
- Ben and Catherine Ivy Foundation
- Jean Perkins Foundation
- National Cancer Institute
- Phelps Family Foundation
- UCLA
- R25T CA098010
- NIH
- Korean-American Scientists and Engineers Association
- Created
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2016-10-31Created from EPrint's datestamp field
- Updated
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2022-06-01Created from EPrint's last_modified field