Low-Temperature Spectroscopy of Biological Compounds
Abstract
It is well substantiated that the absorption spectra of complex molecules may be sharpened, and in many cases the vibrational and rotational details brought out, by refrigeration of the absorbing material to the temperature of liquid nitrogen or lower. This principle seems of great potential utility for the characterization and differentiation of compounds of biological interest heretofore indistinguishable by their absorption characteristics, and conceivably for the solution of cytochemical problems. However, the techniques that have been employed have only limited application to polar compounds. We have therefore developed new techniques with which we have undertaken a study of the absorption spectra of a number of purines, pyrimidines, amino acids, proteins, etc. at the temperature of liquid nitrogen (77° K).
Additional Information
© 1948 American Association for the Advancement of Science. This work was aided by a grant from the American Cancer Society recommended by the National Research Council Committee on Growth.Additional details
- Eprint ID
- 71194
- DOI
- 10.1126/science.107.2777.302
- Resolver ID
- CaltechAUTHORS:20161017-154732901
- American Cancer Society
- Donner Foundation
- Created
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2016-10-18Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field