Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published October 2010 | Published + Accepted Version
Journal Article Open

Integrated Photoacoustic and Fluorescence Confocal Microscopy

Abstract

We have developed a dual-modality imaging system by integrating optical-resolution photoacoustic microscopy and fluorescence confocal microscopy to provide optical absorption and fluorescence contrasts simultaneously. By sharing the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence images are acquired in a single scan. The micrometer resolution allows imaging of both blood and lymphatic vessels down to the capillary level. Simultaneous photoacoustic angiography and fluorescence lymphangiography were demonstrated, presenting more information to study tumor angiogenesis, vasculature, and microenvironments in vivo.

Additional Information

© 2010 IEEE. Manuscript received May 1, 2010; revised June 11, 2010; accepted June 17, 2010. Date of publication July 15, 2010; date of current version September 15, 2010. This work was supported in part by the National Institutes of Health under Grant R01 EB000712, Grant R01 EB008085, Grant R01 CA113453901, Grant U54 CA136398, and Grant 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

Attached Files

Published - 05510116.pdf

Accepted Version - nihms243293.pdf

Files

05510116.pdf
Files (906.0 kB)
Name Size Download all
md5:40d5aa28522017eb8a87cd44bebfed9d
272.3 kB Preview Download
md5:b092e76a2a285a1a1b4cf38d60114561
633.7 kB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 20, 2023