Multifocal optical-resolution photoacoustic microscopy in vivo

Creators: Song, Liang; Maslov, Konstantin; Wang, Lihong V.

Abstract

Although ultrasound arrays have been exploited in photoacoustic imaging to improve imaging speed, ultrasound-array-based optical-resolution photoacoustic microscopy (OR-PAM) has never been achieved previously to our knowledge. Here we present our development of multifocal OR-PAM using a microlens array for optical illumination and an ultrasound array for photoacoustic detection. Our system is capable of imaging hemoglobin concentration and oxygenation in individual microvessels in vivo at high speed. Compared with a single focus, multiple foci reduce the scanning load and increase the imaging speed significantly. The current multifocal system can acquire 1000×500×200 voxels at ∼10 μm lateral resolution within 4 min.

Additional Information

© 2011 Optical Society of America. Received November 23, 2010; revised February 11, 2011; accepted February 27, 2011; posted March 9, 2011 (Doc. ID 138656); published March 29, 2011. This work was sponsored in part by National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, and 5P60 DK02057933. L. Wang has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which did not, however, support this work. The authors appreciate James Ballard's close reading of the manuscript. They also thank Junjie Yao, Lidai Wang, Zijian Guo, and Bin Rao for helpful discussions; Robert Berry for help on machining; and Guo Li for help in preparing the 3D figure of the system.

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