Label-Free Bond-Selective Imaging by Listening to Vibrationally Excited Molecules
Abstract
We report the realization of vibrational photoacoustic (VPA) microscopy using optical excitation of molecular overtone vibration and acoustic detection of the resultant pressure transients. Our approach eliminates the tissue scattering problem encountered in near-infrared spectroscopy and enables depth-resolved signal collection. The 2nd overtone of the CH bond stretch around 8300  cm^(−1), where blood interference is minimal, is excited. We demonstrate 3D VPA imaging of lipid-rich atherosclerotic plaques by excitation from the artery lumen, and lipid storage in live Drosophila larvae, with millimeter-scale penetration depth.
Additional Information
© 2011 American Physical Society. Received 12 November 2010; revised manuscript received 7 May 2011; published 10 June 2011; publisher error corrected 15 June 2011. We acknowledge a grant from the American Heart Association to H.W.W.; support from R01 EB7243 to J. X. C.; HL062552 and the Fortune-Fry Ultrasound Research Fund to M. S.; and R01 EB000712, U54 CA136398, and 5P60 DK02057933 to L.V.W.Errata
Publisher's Note: Label-Free Bond-Selective Imaging by Listening to Vibrationally Excited Molecules [Phys. Rev. Lett. 106, 238106 (2011)] Han-Wei Wang, Ning Chai, Pu Wang, Song Hu, Wei Dou, David Umulis, Lihong V. Wang, Michael Sturek, Robert Lucht, and Ji-Xin Cheng Phys. Rev. Lett. 106, 259901Attached Files
Published - PhysRevLett.106.238106.pdf
Supplemental Material - WangVideo1.avi
Supplemental Material - WangVideo2.avi
Supplemental Material - WangVideo3.avi
Supplemental Material - WangVideo4.avi
Supplemental Material - Wang_Supp_VPA_2rs_to_PRL_final.doc
Supplemental Material - Wang_Supp_VPA_2rs_to_PRL_final.pdf
Erratum - PhysRevLett.106.259901.pdf
Files
Name | Size | Download all |
---|---|---|
md5:cc9a44223560cb8f1e2db870fc07d1ed
|
45.4 MB | Download |
md5:e5f0c016ee96db0697657c9964ec3bc7
|
1.6 MB | Preview Download |
md5:ecaba10cc8cb6b80dcce58b76165f4c8
|
20.2 kB | Preview Download |
md5:a6269413627b3183bbd097069906dfd0
|
45.4 MB | Download |
md5:947e134fc77f9a5bbba256854c91594d
|
2.0 MB | Download |
md5:92628b4a422c582dad93f165811bedc5
|
2.0 MB | Preview Download |
md5:842cff284d2c963aeb0cd7faf196001e
|
45.4 MB | Download |
md5:d2c8d314251ba304d9b36b75dffefaa4
|
45.4 MB | Download |
Additional details
- PMCID
- PMC3398792
- Eprint ID
- 69759
- Resolver ID
- CaltechAUTHORS:20160818-105215538
- American Heart Association
- NIH
- R01 EB7243
- NIH
- HL062552
- Fortune-Fry Ultrasound Research Fund
- NIH
- R01 EB000712
- NIH
- U54 CA136398
- NIH
- 5P60 DK02057933
- Created
-
2016-08-18Created from EPrint's datestamp field
- Updated
-
2021-11-11Created from EPrint's last_modified field