Members of the methanotrophic genus Methylomarinum inhabit inland mud pots
Abstract
Proteobacteria capable of converting the greenhouse gas methane to biomass, energy, and carbon dioxide represent a small but important sink in global methane inventories. Currently, 23 genera of methane oxidizing (methanotrophic) proteobacteria have been described, although many are represented by only a single validly described species. Here we describe a new methanotrophic isolate that shares phenotypic characteristics and phylogenetic relatedness with the marine methanotroph Methylomarinum vadi. However, the new isolate derives from a terrestrial saline mud pot at the northern terminus of the Eastern Pacific Rise (EPR). This new cultivar expands our knowledge of the ecology of Methylomarinum, ultimately towards a fuller understanding of the role of this genus in global methane cycling.
Additional Information
© 2016 Fradet et al. Distributed under Creative Commons CC-BY 4.0. Submitted 25 February 2016; Accepted 18 May 2016; Published 12 July 2016. We thank Roland Hatzenpichler and Derek Smith for sampling the Salton Sea mudpots in 2012 and providing critical methodological, graphic, and scientific input.We thank Alyssa Boedigheimer for assistance in sample collection in January 2016. We thank Stephanie Connon for assistance with the 16S rRNA gene phylogeny. Funding for this work was provided by the Gordon and Betty Moore Foundation, in a grant to Victoria J. Orphan (grant no. GBMF3780). This research was additionally supported by a grant from the NASA Astrobiology Institute (Award # NNA13AA92A). This is NAI-Life Underground Publication Number 083. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Grant Disclosures: The following grant information was disclosed by the authors: Gordon and Betty Moore Foundation, in a grant to Victoria J. Orphan: GBMF3780. NASA Astrobiology Institute, in a grant to Victoria J. Orphan: NNA13AA92A. The authors declare that they have no competing interests. Author Contributions: Danielle T. Fradet conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper. Patricia L. Tavormina conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper. Victoria J. Orphan conceived and designed the experiments, wrote the paper, reviewed drafts of the paper, intellectual Support, encouragement, and funding.Attached Files
Published - peerj-2116.pdf
Supplemental Material - Figure_S1.jpg
Supplemental Material - Figure_S2.jpg
Supplemental Material - Figure_S3.jpg
Supplemental Material - Movie_S1.mov
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Additional details
- PMCID
- PMC4950536
- Eprint ID
- 69572
- Resolver ID
- CaltechAUTHORS:20160811-151334696
- Gordon and Betty Moore Foundation
- GBMF3780
- NASA
- NNA13AA92A
- Created
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2016-08-11Created from EPrint's datestamp field
- Updated
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2023-06-02Created from EPrint's last_modified field
- Caltech groups
- Division of Geological and Planetary Sciences (GPS)