Photoacoustic recovery after photothermal bleaching in living cells

Creators: Li, Chiye; Zhang, Chi; Gao, Liang; Garcia-Uribe, Alejandro; Wang, Lihong V.

Abstract

We present an innovative method, photoacoustic recovery after photothermal bleaching (PRAP), for studying particle dynamics at micron scale via photoacoustic imaging. As an intuitive way to visualize and quantify dynamic processes, PRAP is demonstrated first in a simple phantom study and then in a more complex measurement involving live cells. Compared with the conventional fluorescence-based approach, PRAP provides high signal-to-noise ratio (SNR) imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic processes inside a cell.

Additional Information

© 2013 SPIE. Paper 130271RR received Apr. 22, 2013; revised manuscript received Aug. 28, 2013; accepted for publication Sep. 6, 2013; published online Oct. 2, 2013. This work was sponsored in part by National Institutes of Health (NIH) grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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