Published January 1, 2002
| public
Journal Article
Improvement in the Apparent Mass Resolution of Oligonucleotides by Using ^(12)C/^(14)N-Enriched Samples
Chicago
Abstract
The apparent mass resolution of oligonucleotides in time-of-flight (TOF) mass spectrometers has been examined. In a reflectron TOF instrument, where the isotopic profile can be completely resolved, the apparent resolution matches the instrument's resolving power. In a linear TOF instrument, unresolved isotopic profiles limit the apparent resolution to much lower values than the actual instrument resolution. By using ^(12)C/^(14)N-enriched oligonucleotides, the apparent resolution can be improved significantly. The isotope enrichment method also enhances the signal-to-noise ratio.
Additional Information
© 2002 American Chemical Society. Received 17 July 2001; accepted 25 October 2001; published online 1 December 2001; published in print 1 January 2002. We thank Dr. Norman Chiu, Dr. Dirk van den Boom, and Professor Franz Hillenkamp for discussions; Dr. Ralf Wörl and Dr. Michael Willis for advice on RNA purification for MALDI; and Prof. Catherine Fenselau for encouragement of this work. This work was supported in part by a grant from the NIH (GM-53320 to J.R.W.).Additional details
- Eprint ID
- 69423
- Resolver ID
- CaltechAUTHORS:20160803-155304867
- NIH
- GM-53320
- Created
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2016-08-03Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field