In vivo methylation of replicating bacteriophage φX174 DNA
Abstract
The pattern of DNA methylation during the infection of Escherichia coli C cells with bacteriophage φX174, has been studied. In vivo methylated DNA was isolated and analyzed using the following techniques: velocity sedimentation through neutral and alkaline sucrose gradients, isopycnic analysis, chromatography on benzoylated DBAE-cellulose columns and specific enzymatic digestion. All these analytical methods indicated that the DNA molecules that are methylated during the process of phage φX DNA replication are the replicating intermediates composed of a circular complementary strand and a viral strand larger than one genome length. It is concluded that methylation occurs on the nascent DNA strand of the replicating intermediates involved in the synthesis of progeny single-stranded DNA.
Additional Information
© 1973 Elsevier Ltd. Received 19 December 1972, Revised 11 April 1973. This research was supported in part by a grant from the U.S. Public Health Service, GM13554, and a postdoctoral fellowship, 1F05 TW1348 to one of us (A.R.). A part of this study was carried out in the Department of Cellular Biochemistry. The Hebrew University-Hadassah Medical School, Jerusalem, Israel, and supported by the Joint Research Fund of the Hebrew University and Hadassah.Additional details
- Eprint ID
- 69414
- Resolver ID
- CaltechAUTHORS:20160803-123956450
- GM 13554
- U.S. Public Health Service (USPHS)
- 1F05 TW1348
- NIH Postdoctoral Fellowship
- Hebrew University
- Hadassah Medical School
- Created
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2016-08-04Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field