Published February 1, 2006
| Supplemental Material
Journal Article
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Parallel Picoliter RT-PCR Assays Using Microfluidics
Chicago
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Abstract
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.
Additional Information
© 2006 American Chemical Society. Received for review August 3, 2005. Accepted November 24, 2005. Publication Date (Web): January 4, 2006. The authors thank Kathy Burke, Sebastian Maerkl, and Carl Hansen for helpful discussions. This work was supported in part by a National Research Service Award (T32GM07616) from the National Institute of General Medical Sciences (J.S.M.) and National Institutes of Health (NIH) Grant NIH 1RO1 HG002644-01A1.Attached Files
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Additional details
- Eprint ID
- 69384
- DOI
- 10.1021/ac0513865
- Resolver ID
- CaltechAUTHORS:20160802-102718412
- NIH Predoctoral Fellowship
- T32GM07616
- NIH
- 1RO1 HG002644-01A1
- Created
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2016-08-02Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field