Ultraviolet Sensitivity of the Biological Activity of ΦX174 Virus, Single-Stranded DNA, and RF DNA
- Creators
- Yarus, Michael
- Sinsheimer, Robert L.
Abstract
Action spectra for inactivation of ϕX virus, free ϕX single-stranded DNA, and double-stranded ϕX DNA (RF) have been measured using light of wavelength 225-302 mμ. The sensitivity of RF has been determined using bacterial hosts both capable and incapable of reactivation of UV damage. The inactivation of ϕX virus is due, at all wavelengths, to damage to its DNA; it appears that, below 240 mμ, energy absorbed by viral structural protein may inactivate the viral DNA. The variation of the probability of inactivation by an absorbed quantum (quantum yield) with wavelength, in the case of free-single-stranded ϕX DNA, suggests that energy absorbed by pyrimidine residues is more likely to yield inactivation than absorption by purines. This implies that energy transfer is not so extensive as to make all absorbed energy available to pyrimidines.
Additional Information
© 1967 Biophysical Society. Received for publication 9 November 1966. This work is drawn from a thesis by M. Yarus submitted in partial fulfillment of the requirements for the Ph. D. degree. We would like to thank Max Delbruck for the use of the monochromator, which he and his group had calibrated and made usable. This work was supported, in part, by USPHS Fellowship 1F1 GM21,328-01 and by grants RG 6965 and GM 13554 from the U. S. Public Health Service.Additional details
- PMCID
- PMC1368031
- Eprint ID
- 69248
- Resolver ID
- CaltechAUTHORS:20160727-102724849
- 1F1 GM21, 328-01
- U.S. Public Health Service (USPHS)
- RG 6965
- NIH
- GM 13554
- U.S. Public Health Service (USPHS)
- Created
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2016-07-27Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field