Near-infrared optical-resolution photoacoustic microscopy
Abstract
Compared with visible light (380–700 nm), near-infrared light (700–1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm^2). Beyond ∼0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.
Additional Information
© 2014 Optical Society of America. Received May 9, 2014; revised July 25, 2014; accepted August 1, 2014; posted August 4, 2014 (Doc. ID 211735); published August 28, 2014. The authors appreciate Prof. James Ballard's close reading of the manuscript. This work was sponsored by NIH Grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA186567 (NIH Director's Transformative Research Award), and R01 CA159959 and NSF Grant 1255930. L. W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc. which, however, did not support this work. K. M. has a financial interest in Microphotoacoustics, Inc.Attached Files
Published - ol-39-17-5192.pdf
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Additional details
- PMCID
- PMC4161671
- Eprint ID
- 69133
- Resolver ID
- CaltechAUTHORS:20160720-103159834
- NIH
- DP1 EB016986
- NIH
- R01 CA186567
- NIH
- R01 CA159959
- NSF
- DBI-1255930
- Created
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2016-07-25Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field