Deep-Tissue Photoacoustic Tomography of a Genetically Encoded Near-Infrared Fluorescent Probe

Creators: Filonov, Grigory S.; Krumholz, Arie; Xia, Jun; Yao, Junjie; Wang, Lihong V.; Verkhusha, Vladislav V.

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Abstract

Skin-deep: The combination of a near-infrared fluorescent protein (iRFP) and deep-tissue photoacoustic tomography clearly demonstrates the superiority of iRFP over other genetically encoded probes. Impressive resolution (280 μm lateral and 75 μm axial) was obtained at a depth of 4 mm in a live animal, and volumetric images of a tumor were produced (see image), thus allowing the spatially resolved monitoring of its development.

Additional Information

© 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: October 5, 2011; Published online: December 23, 2011. We thank Bojana Gligorijevic for help with the mice, and Jeffrey Segall for providing the EGFP-expressing MTLn3 cells. We are grateful to Benjamin Glick (University of Chicago), Dmitry Chudakov and Konstantin Lukyanov (both from the Institute of Bioorganic Chemistry, Russia) for the plasmids encoding GFP-like proteins. We thank Yu Zhang and Yunan Xia for help with maintaining cell cultures. We thank James Ballard for help with editing the manuscript. This work was supported by the National Institutes of Health grants GM073913 and CA164468 (to V.V.V.), and in part by EB000712, EB008085, CA134539, and CA136398 (to L.V.W.).

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Accepted Version - nihms-356402.pdf

Supplemental Material - anie_201107026_sm_miscellaneous_information.pdf

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