In vivo photoacoustic tomography of myoglobin oxygen saturation

Creators: Lin, Li; Yao, Junjie; Li, Lei; Wang, Lihong V.

Abstract

Myoglobin is an essential oxygen-binding hemoprotein in skeletal and cardiac muscles that buffers intracellular oxygen (O_2) concentration in response to hypoxia or elevated muscle activities. We present a method that uses photoacoustic computed tomography to measure the distribution of myoglobin in tissue and the oxygen saturation of myoglobin (sO_2-Mb). From photoacoustic measurements of mice in different oxygenation states, we performed calibration-free quantification of the sO_2 –Mb change in the backbone muscle in vivo.

Additional Information

© 2015 SPIE. Paper 150407SSR received Jun. 16, 2015; accepted for publication Sep. 11, 2015; published online Dec. 17, 2015. The authors would like to thank Professor James Ballard for close reading of this paper. We also thank Guo Li, Dr. Junhui Shi, and Dr. Lidai Wang for useful discussions. This work was supported in part by the National Institutes of Health Grants Nos. DP1 EB016986 (NIH Director's Pioneer Award), R01 CA186567 (NIH Director's Transformative Research Award), and R01 EB016963. L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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