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Published April 1966 | public
Journal Article

The Process of Infection with Bacteriophage ΦX174: IX. Studies on the Physiology of Three ΦX174 Temperature-sensitive Mutants

Abstract

Representatives of three different complementing groups of ΦX174 temperature-sensitive mutants were studied for their ability to synthesize infective DNA at the restrictive temperature. One of the mutants showed normal or nearly normal production of infective single-stranded DNA (when compared with wild-type phage), whereas the other two failed to make any substantial amount. Double-stranded DNA components, of two densities in alkali, were made in all infections.

Additional Information

© 1966 Elsevier Ltd. Received 16 September 1965, and in revised form 26 December 1965. We acknowledge the excellent technical assistance of Miss Alma Shafer. One of us (C.E.D.) was a postdoctoral fellow of the National Institutes of Health. This research was supported, in part, by research grant RG 6965 from the U.S. Public Health Service.

Additional details

Created:
August 19, 2023
Modified:
October 19, 2023