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Published July 1966 | public
Journal Article

The Process of Infection with Bacteriophage ΦX174: X. Mutations in a ΦX Lysis Gene

Abstract

The ability of conditional lethal mutants of phage ΦX174 to induce host cell lysis during infection under restrictive conditions has been studied. We have found amber (am) and temperature-sensitive (ts) mutants which present a variety of alterations in the normal lytic process. In particular, there is a class of am mutants which do not produce cell lysis but otherwise replicate normally in the restrictive host. These mutants constitute a single complementation group. The existence of these mutants implicates a phage-coded protein in the lytic process. This protein is not an essential structural component of the phage, since normal phage particles are produced in the absence of lysis.

Additional Information

© 1966 Elsevier Ltd. Received 12 January 1966. We thank D. Pfeifer, S. G. Krane, M. Yarus, R. Levine, and G. Streisinger for permission to mention unpublished results. We also wish to acknowledge the capable technical assistance of Alma Shafer and Marianne Lawrence. This work was supported in part by grants RG6965 and GM13554 of the U.S. Public Health Service.

Additional details

Created:
August 19, 2023
Modified:
October 19, 2023