The process of infection with bacteriophage φX174: IV. Replication of the Viral DNA in a Synchronized Infection

Creators: Denhardt, David T.; Sinsheimer, Robert L.

Abstract

An investigation has been made of the origin and mode of replication of viral specific nucleic acids during the replication of bacteriophage φX174. The DNA combining with the infecting single strand to form the first replicative form is synthesized after infection from low molecular weight precursors from the medium. The rate of increase in the amount of infective replicative form in the culture during the eclipse period is constant, and the replicative form molecule containing the parental single strand replicates semi-conservatively. The conserved subunit is a single polynucleotide strand. The material constituting the progeny single-strand DNA is derived directly from the medium, not from the replicative form.

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© 1965 Elsevier Ltd. Received 29 December 1964, and in revised form 22 March 1965. This work was reported in a thesis submitted by one of us (D.T.D.) to the California. Institute of Technology in partial fulfillment of the requirements for the degree of Doctor of Philosophy, and was supported in part by fellowships from the U.S. National Science Foundation and the U.S. Public Health Service. This research was also supported, in part, by a grant, RG 6965, from the U.S. Public Health Service. The kind assistance given by Dr George Guthrie in the early phases of this work, especially with regard to the protoplast assay technique, was very much appreciated. The technique of making Bu-substituted φX was first developed by Dr Thomas Thompson, and his pioneering work is acknowledged with pleasure. Discussions with Dr Alice Burton, Dr E. Carusi and Mr Michael Yarus materially aided the progress of this work.

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The process of infection with bacteriophage φX174: IV. Replication of the Viral DNA in a Synchronized Infection

Abstract

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