Published August 1964
| public
Journal Article
Structure of the DNA of Bacteriophage ΦX174: V. Purine Sequences
- Creators
- Sedat, John
- Sinsheimer, Robert L.
Chicago
An error occurred while generating the citation.
Abstract
Methods are described for the preparation of DNA, specifically labeled with carbon-14 in the purines, from bacteriophage ΦX174. This DNA, mixed with unlabeled calf thymus DNA as a carrier, has been degraded to purine tracts by hydrazinolysis and subsequent alkaline hydrolysis. The purine tracts have been separated according to magnitude by chromatography on a column of DEAE-Sephadex, at 55°C in the presence of 7 M-urea.
Additional Information
© 1964 Elsevier Ltd. . , Available online 15 May 2009. We acknowledge the assistance of Mrs. Regine Weil in the growth and purification of the purine-labeled ΦX and of Mrs. Carolyn Nagler in the chromatographic refractlonations, This research was supported, in part, by a grant, RG6965, from the U.S. Public Health Service.Additional details
- Eprint ID
- 68455
- Resolver ID
- CaltechAUTHORS:20160617-071022953
- U.S. Public Health Service (USPHS)
- RG6965
- Created
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2016-06-17Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field