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Published March 1963 | public
Journal Article

The Replication of Bacteriophage MS2: 1. Transfer of Parental Nucleic Acid to Progeny Phage

Abstract

Conditions and procedures are described for the growth and assay of bacteriophage MS2, an RNA-containing phage. Procedures are described for the preparation of ^(15)N^(32)P-labeled MS2 phage, and for the use of such labeled phage in the measurement of transfer of parental RNA to progeny phage. No evidence for any type of conservative or semi-conservative transfer is found and dispersive transfer is limited to 3%.

Additional Information

© 1963 Elsevier Ltd. Received 20 November 1962. The able technical assistance of Mrs. Shizuko Yagi is gratefully acknowledged. One of us (J.E.D.) is a Postdoctoral fellow of the U.S. Public Health Service. This research was supported in part by a grant from the U.S. Public Health Service No. RG6965.

Additional details

Created:
August 19, 2023
Modified:
October 19, 2023