Published February 1963
| public
Journal Article
The structure of the DNA of bacteriophage φX174: IV. Pyrimidine Sequences
- Creators
- Hall, John B.
- Sinsheimer, Robert L.
Abstract
Apurinic acid was prepared from ^(14)C-labeled DNA of the bacteriophage φX174 with unlabeled calf thymus DNA as carrier. The terminal phosphates of the pyrimidine tracts were removed by treatment with the alkaline phosphatase of E. coli and the resulting mixture fractionated on a column of DEAE-Sephadex into fragments of uniform chain length (isopliths) up to undecanucleotides.
Additional Information
© 1963 Elsevier Ltd. Received 26 September 1962. We wish to thank Dr. I. R. Lehman for his generous gift of E. coli phosphodiesterase. We would also like to express our appreciation to Miss Kathleen Gaines and Mr. John Sedat for their excellent assistance. This research was supported, in part, by U.S. Public Health Service Grants RG6965 and H3103.Additional details
- Eprint ID
- 67978
- DOI
- 10.1016/S0022-2836(63)80127-8
- Resolver ID
- CaltechAUTHORS:20160616-133842222
- RG6965
- U.S. Public Health Service (USPHS)
- H3103
- U.S. Public Health Service (USPHS)
- Created
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2016-06-17Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field