Replication of Sindbis Virus I. Relative Size and Genetic Content of 26 s and 49 s RNA
- Creators
- Simmons, Daniel T.
- Strauss, James H.
Abstract
The genome of Sindbis virus, 49 s RNA, is a single, intact polynucleotide chain having a molecular weight of 4.3±0.3 x 10^6 daltons. This has been determined using a variety of methods including polyacrylamide gel electrophoresis, sedimentation after reaction with formaldehyde, determination of the molecular weights of the double-stranded forms of Sindbis-specific RNA and hybridization competition. The second major species of single-stranded RNA made after infection with Sindbis, 26 s RNA, has been found to have a molecular weight of 1.6 x 10^8 daltons as determined by sedimentation in dimethylsulfoxide. Hybridization-competition experiments carried out between these two species of RNA, using double-stranded forms of Sindbis RNA isolated from infected cells, showed that 26 s RNA contains only one-third of the base sequences in 49 s RNA and thus represents a unique fraction of the viral genome.
Additional Information
© 1972 Elsevier Ltd. Received 14 February 1972, and in revised form 10 July 1972. We are pleased to acknowledge the expert technical assistance of Edith M. Lenches and Mildred Quisenberry Hoover. We thank Dr. Ellen G. Strauss and John S. Pierce for their helpful editorial advice for the construction of this manuscript. This investigation was supported in part by the National Science Foundation grant no. GB13508 and by the U.S. Public Health Service grant no. GM06965. One of us (D. T. S.) was supported by the National Institutes of Health training grant no. 5-Tl-GM86.Additional details
- Eprint ID
- 67889
- DOI
- 10.1016/S0022-2836(72)80026-3
- Resolver ID
- CaltechAUTHORS:20160613-145752389
- GB13508
- NSF
- GM06965
- NIH
- 5-T1-GM86
- NIH Predoctoral Fellowship
- Created
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2016-06-13Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field