Purification and properties of bacteriophage φX174
- Creators
- Sinsheimer, Robert L.
Abstract
Procedures are described for the isolation in pure form of the bacterial virus φX174. This virus is shown to have a particle weight of 6·2 × 10^6 and to contain 25 % by weight of DNA. At neutral pH, the purified virus forms a discrete aggregate, probably a tetra-mer. This aggregate dissociates either upon dilution or at alkaline pH. Lysates produced by φX174 contain a second particle, antigenically related to the virus, but not infective and of lower sedimentation rate and lesser DNA content. The DNA of φX174 appears to have an unusual structure in that it reacts with formaldehyde (even before extraction from the virus) and in that the atomic efficiency of inactivation of the virus by decay of incorporated phosphorus-32, calculated by combining our data and that of Tessman, is 1·0.
Additional Information
© 1959 Elsevier Ltd. Received 4 November 1958. Received 4 November 1958. This work was initiated while the author was on the staff of the Physics Department of Iowa State College. This research has been supported by grants from the U.S. Public Health Service, the American Cancer Society and the California Division of the American Cancer Society. It is a pleasure to indicate the technical assistance, at various times, of Mrs. Jean Sonder, Mrs. Alice White, Mrs. Agnes Gara, Mrs. Betsy Laties, Miss Faith Poole, and Miss Sharon Palmer. Antigenic studies of the 70S particles were made by Mr. George Guthrie. Drs. M. Meselson and J. Vinograd gave valuable assistance in the initial use of the density gradient sedimentation equilibrium method.Additional details
- Eprint ID
- 67453
- DOI
- 10.1016/S0022-2836(59)80005-X
- Resolver ID
- CaltechAUTHORS:20160527-120815417
- U.S. Public Health Service (USPHS)
- American Cancer Society, California Division
- Created
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2016-05-27Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field