Message sequences and short repetitive sequences are interspersed in sea urchin egg poly(A)^+ RNAs

Creators: Costantini, Franklin D.; Britten, Roy J.; Davidson, Eric H.

Abstract

At least half of the mass and most of the different single-copy maternal mRNA sequences in the egg are covalently associated with transcripts of short repetitive sequences. Only a restricted group of the diverse genomic repeat families are significantly represented. The messages fall into several hundred sets, each containing transcripts from a different repeat family.

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© 1980 Nature Publishing Group. Received 13 March; accepted 20 June 1980. We thank Drs William H. Klein and Richard H. Scheller for their assistance and Drs Richard Axel and Norman Davidson for critical reviews of the manuscript. This research was supported by NIH Grant HD-05753. R. J. B. is a staff member of the Carnegie Institution of Washington. Note added in proof: The characteristics of sea urchin egg p(A)^+ RNA described here pertain as well to the p(A)^+ RNA of Xenopus, an anuran vertebrate. When p(A)^+ RNA from the unfertilized egg of Xenopus (obtained by Dr L. Dennis Smith) was renatured and spread for electron microscopy under the exact conditions of Fig. 2 (by M. E. Chamberlin), the following results were obtained. (1) RNA which was not permitted to anneal after denaturation displayed only occasional intrastrand foldback structures. (2) RNA renatured to C_0t 60 was largely involved in structures containing two to four molecules bound by short ,duplex regions. And (3), RNA renatured to C_0t 600 was largely included in complex multimolecular aggregates often containing more than 10 molecules.

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Message sequences and short repetitive sequences are interspersed in sea urchin egg poly(A)^+ RNAs

Abstract

Additional Information

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